To read more about PRIMER3 and penalty score, follow the link below. ![]() Deviations from the optimum primer size and Tm have a large influence on the penalty score. Lower penalty score indicates a better primer pair. Q Value : This is a PRIMER3 penalty score that indicates deviation from the specified optimal design parameters. ![]() GC clamp : Required number of G or C bases at the 3' of the left and right external primers.
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